Before a nanoparticle is going to be used both in vitro and in vivo, it is necessary to characterize, scientifically proven, their intrinsic properties such as size, monodispersity, aqueous stability, net charge, ability of nonspecific adsorption of proteins, aggregation and flocculation in biological media etc.. This information is vital to know whether a nanoparticle can be used in a particular biological application, or else to improve their synthetic process, and / or chemical functionalization.

Another challenge in using NPs on Medicine and Biotechnology is the great variability in manufacturing methods, materials used, and scale necessary to produce adequate volumes of uniform nanoparticles. Today there is no systematic and rigorous monitoring of NPs production and its variability batch to batch. This sometimes makes it difficult to compare results obtained by different research groups. There is therefore a real need to create a classification scheme, systematic and consistent, of nanoparticles where their physicochemical properties can be reflected.

 

In this context, in addition to having staff and scientific advisors with extensive experience, nanoimmunotech can access state of the art equipment in characterization of nanostructured materials.

CHARACTERIZATION	TECHNIQUE
Size, Shape, Monodispersity, Composition	TEM-EDX
Content analysis of C, N, H and S.	Analysis of elements
Protective organic monolayer structure of nanoparticles core-shell type	Nuclear magnetic resonance spectroscopy (NMR)
Identification and quantification of the chemical elements present in the NPS.	ICP
Surface charge of nanoparticles.	Zeta potential
Hydrodynamic radius and size distribution  of particles in solution.	Dynamic light scattering (DLS)
Characterization of the major functional groups present on the surface of the NPS.	Fourier transform infrared Spectrophotometry(FTIR)
Assessing the nature and chemical state of the surface atomsof the NPs to a depth of 5 nm (4-20 atomic layers).	X-ray photoelectron spectroscopy (XPS)
Analyze fluorescence of NPs.	Fluorescence spectrophotometry
Characterization of surface plasmon of gold NPs.	UV-visible spectroscopy

 

Previous experience of nanoimmunotech team:

Magnetic nanoparticles for power absorption: Optimizing size, shape and magnetic properties. .
Gonzalez-Fernandez MA, Torres TE, Andres-Verges M, Costo R, de la Presa R, Serna CJ, Morales MR, Marquina C, Ibarra MR, Goya GF
Journal of Solid State Chemistry  (2009), 182(10), 2779-2784.

Single-step chemical synthesis of ferrite hollow nanospheres.
Lima E, Vargas JM, Zysler RD, Rechenberg HR, Cohen Rn, Arbiol J, Goya GF, Ibarra A, Ibarra MR
Nanotechnology (2009) 30(4),  Art. Nº045606.

Structural, magnetic and electronic properties of surface oxidised Fe nanoparticles.
Przewoznik J, Tyliszczak T Rybicki D, Zukrowski J, Szczerba W, Sikora M, Kapusta C, Stepankova H, Pacheco RF), Serrate D, Ibarra MR
Perspectives of Nanoscience and Nanotechnology-Acta Amterialia Gold Medal Workshop. Book Series: Solid State Phenomena (2008), 140, 47-54.

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